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Single particle characterisation in biologics: from mid-infrared micro-spectroscopy and mapping to spectral imaging
The presence of “particles” in protein pharmaceuticals (biologics) can cause severe, unwanted effects in the drug. The article describes the use of mid-infrared micro-spectroscopy for the investigation and chemical characterisation of single particles in these biologics.
This Product Focus is on Raman Spectroscopy; a number of companies have provided information on their key products, their applications and features.
Reverse engineering of polymeric multilayers using AFM-based nanoscale IR spectroscopy and thermal analysis
It is possible to obtain both infrared spectra and thermal analysis data of individual layers in a cross-sectioned multilayer film. Since both techniques are AFM-based, the topographical features can be readily linked to the spectroscopic and thermal data at a much higher spatial resolution than previously achievable.
RM21 is a precision aligned microscope platform designed for improved user accessibility by Mad City Labs and distributed by Elliot Scientific. It offers microscopists the opportunity to develop flexible configuration instruments with ease. It is precision manufactured to allow easy alignment of microscopy and optical components within its three-dimensional space and all posts and fixturing points are referenced to a known datum. With a robust design, precision manufacturing and assembly, it is a suitable platform for a range of microscopy applications including super resolution microscopy, fluorescence microscopy and TIRF. It includes a precision platform and an axial motorised Z-axis suitable for lens positioning. An encoder option is also available.
Anasys Instruments has added arbitrary polarisation control to their nanoIR nanoscale IR spectroscopy system which enables users to measure and visualise molecular orientation with nanoscale spatial resolution which is particularly useful in the study of polymeric fibres where molecular orientation is important to controlling their properties.
UV/vis/NIR microscope based on a double-beam scanning spectrophotometer capable of covering 200–2700 nm. There are three different versions: one for the UV/vis, one with a Peltier-cooled detector for the entire 200–2700 nm range and one with an InGaAs detector for measurements between 200 nm and 1700 nm. All models have a user-selectable slit width for variable resolution, as well as selectable circular apertures and an adjustable rectangular aperture for sample area discrimination.
A new 3D fast imaging capability for the inVia Raman microscope. The inVia systems—with the StreamLineHR™ imaging option—can now collect and display Raman data from within transparent materials. This provides users with full 3D visualisation of their samples.
Nati Salvadó, Salvador Butí and Trinitat Pradell have used a number of techniques to investigate changes in pictorial techniques in Catalan paintings in the 15th century. The combination of different techniques is of particular value. The use of synchtrotron radiation as a light source is also an advantage.
Information on the detailed chemical composition, structure and morphology of environmental particles, and especially airborne particulate matter (PM), facilitate the understanding of their reactivity, sources, transport and changes of chemical species and, hence, prediction of their likely impact on the environment and human and animal health. The analysis techniques for environmental particles can broadly be divided into two groups: bulk (for example, water-soluble ionic content by means of ion chromatography for PM, elemental concentrations by means of X-ray fluorescence spectrometry for all environmental particles, chemical structural information by means of X-ray diffraction for larger environmental particles, such as sediments and sands etc.) and micro-analytical techniques, whereby the character of any single particle can be probed.
Bruker to Acquire Veeco's Scanning Probe Microscopy and Optical Industrial Metrology Scientific Instruments Business
Bruker Corporation has signed an agreement to acquire the Scanning Probe Microscopy (SPM) and Optical Industrial Metrology (OIM) instruments business from Veeco Instruments, Inc. for $229 million in cash. The transaction has been approved by the Boards of Directors of both companies and is expected to close during the fourth quarter of 2010, pending regulatory review and subject to customary closing conditions.
Revealing the presence of creatine in human spinal cord in amyotrophic lateral sclerosis, by infrared microspectroscopy
We have previously investigated the topographic and quantitative changes in the distribution of trace metals in spinal cords from ALS and control patients. X-ray fluorescence microscopy was used to investigate their metallic nature and distribution in single nerve cells. A deeper understanding of the neurodegenerative processes in ALS requires focus on the biochemical changes occurring in nervous tissue of such a disorder. For this purpose, we have undertaken an infrared microspectroscopy study. While metals are suggested to play a pivotal role in the pathogenesis of ALS, they typically do not occur in tissues as free ions. This results in the presence of the complex mechanisms of metal ions buffering that protect cells against their toxic effects. Metal homeostasis is regulated by several proteins. Such proteins containing metal cofactor are called metalloproteins.
Microscopy with atomic resolution could be useful in the determining the structure of some unknown organic compounds, such as medicinally important natural products, according to a study online in Nature Chemistry. This method could avoid the lengthy and expensive process of trying to synthesise the compound and then compare its structure with that of the natural one, which is necessary in some cases.
WITec has introduced a line extension of the alpha300 microscope series. Building on the system's inherent modularity, several new microscope versions have been added in order to meet all diverse and multi-faceted customer requirements.
Scientists from Ruhr-Universität Bochum have used Raman micro-spectroscopy to distinguish, within seconds, healthy fertile and infertile sperm cells. The method has the potential for a novel fertility technology and a test scheme which does not only rely on morphological characteristics, but also utilises chemical signatures.
The location of metal complexes within living cancer cells has been accurately determined using Raman microscopy. The researchers have thus gained new insights into the mechanism of action of metal-containing drugs, to which they ascribe great potential capacities, e.g. in the treatment of cancer. These findings are of fundamental significance and are thus featured as a VIP (very important paper) in the current edition of Angewandte Chemie.
Espinosa Alonso, a chemist from Utrecht University, The Netherlands, used four different spectroscopic techniques to study catalysts in the course of their preparation: UV-vis-NIR-microspectroscopy, IR microspectroscopy, magnetic resonance imaging (MRI) and tomographic energy dispersive diffraction imaging (TEDDI). Whilst MRI and TEDDI are already frequently used in other research fields, but they are not commonly used to study the preparation of catalysts.
Renishaw has enhanced the capabilities of its inVia microscope which now offers remote system diagnosis and control of key system functions such as laser wavelength change, rapid switching between viewing modes, automated system optimisation and validation, the ability to queue multiple measurements and measurement templates that enable non-expert users to apply identical experimental conditions continuously. The microscopes now offer complete automation, remote control, large area imaging and faster operation.Renishaw Plc Issue: 21/05 RSN: 118 More information via
The National Physical Laboratory (NPL), the UK's National Measurement Institute, is developing equipment and techniques to support the growing use of organic electronics. The market for organic, or plastic, electronics is expected to be worth £15 billion by 2015 (IDTechEx), and NPL is seeking to ensure the infrastructure is in place to allow businesses to achieve commercial success in this emerging area.
WITec has established the WITec Academy as a new forum for advanced instrument and software operation training. It offers a variety of basic and expert level courses covering the entire range of WITec products and potential applications. www.witec.de/en/witecacademy